Second-degree burns with six etiologies treated with autologous noncultured cell-spray grafting

Burns. 2016 Nov;42(7):e99-e106. doi: 10.1016/j.burns.2016.02.020. Epub 2016 Aug 25.


Partial and deep partial-thickness burn wounds present a difficult diagnosis and prognosis that makes the planning for a conservative treatment versus mesh grafting problematic. A non-invasive treatment strategy avoiding mesh grafting is often chosen by practitioners based on their clinical and empirical evidence. However, a delayed re-epithelialization after conservative treatment may extend the patient's hospitalization period, increase the risk of infection, and lead to poor functional and aesthetic outcome. Early spray grafting, using non-cultured autologous cells, is under discussion for partial and deep partial-thickness wounds to accelerate the re-epithelialization process, reducing the healing time in the hospital, and minimizing complications. To address planning for future clinical studies on this technology, suitable indications will be interesting. We present case information on severe second-degree injuries after gas, chemical, electrical, gasoline, hot water, and tar scalding burns showing one patient per indication. The treatment results with autologous non-cultured cells, support rapid, uncomplicated re-epithelialization with aesthetically and functionally satisfying outcomes. Hospital stays averaged 7.6±1.6 days. Early autologous cell-spray grafting does not preclude or prevent simultaneous or subsequent traditional mesh autografting when indicated on defined areas of full-thickness injury.

Keywords: Burns; Cell-spray grafting; Keratinocytes; Re-epithelialization; Wound healing.

Publication types

  • Case Reports

MeSH terms

  • Adolescent
  • Adult
  • Arm Injuries / therapy*
  • Burns / therapy*
  • Burns, Chemical / therapy
  • Burns, Electric / therapy
  • Cell Separation / methods*
  • Endopeptidases
  • Hand Injuries / therapy*
  • Humans
  • Keratinocytes / transplantation*
  • Re-Epithelialization*
  • Skin Transplantation / methods*
  • Thoracic Injuries / therapy*
  • Transplantation, Autologous
  • Trypsin
  • Wound Healing


  • Endopeptidases
  • Trypsin
  • dispase