Identifying Regions Enriched in a ChIP-seq Data Set (Peak Finding)

Jui-Hung Hung; Zhiping Weng

doi:10.1101/pdb.prot093187

Identifying Regions Enriched in a ChIP-seq Data Set (Peak Finding)

Cold Spring Harb Protoc. 2017 Feb 1;2017(2). doi: 10.1101/pdb.prot093187.

Authors

Jui-Hung Hung, Zhiping Weng

PMID: 27574193
DOI: 10.1101/pdb.prot093187

Abstract

This protocol demonstrates how to use MACS software to call peaks in a ChIP-seq data set. MACS is one of the most widely used peak-calling programs. The protocol describes how to run MACS in a UNIX environment and on Galaxy and how to extract the sequences of ±50 bp around the summits of the 500 MACS peaks with the most significant P values.

MeSH terms

Computational Biology / methods*
Gene Expression Profiling / methods*
Software