Site-Specific Labeling of MicroRNA Precursors: A Structure-Activity Relationship Study

Chembiochem. 2016 Nov 3;17(21):2012-2017. doi: 10.1002/cbic.201600370. Epub 2016 Sep 23.


Functionalized oligoribonucleotides are essential tools in RNA chemical biology. Various synthetic routes have been developed over recent years to conjugate functional groups to oligoribonucleotides. However, the presence of the functional group on the oligoribonucleotide backbone can lead to partial or total loss of biological function. The limited knowledge concerning the positioning of functional groups therefore represents a hurdle for the development of oligoribonucleotide chemical tools. Here we describe a systematic investigation of site-specific labeling of pre-miRNAs to identify positions for the incorporation of functional groups, in order not to hinder their processing into active mature miRNAs.

Keywords: RNA recognition; functional groups; gene expression; inhibitors; nucleobases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • MicroRNAs / chemistry*
  • MicroRNAs / metabolism
  • Molecular Structure
  • Oligoribonucleotides / chemical synthesis
  • Oligoribonucleotides / chemistry*
  • Oligoribonucleotides / metabolism
  • Structure-Activity Relationship


  • MicroRNAs
  • Oligoribonucleotides