Lipid droplets are organelles found in most mammalian cells, as well as in various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol in which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation. © 2016 by John Wiley & Sons, Inc.
Keywords: cell cultures; density-gradient centrifugation; isolation of lipid droplets; mammalian; preparation of lipid droplets by centrifugation.
Copyright © 2016 John Wiley & Sons, Inc.