Molecular signatures associated with ZIKV exposure in human cortical neural progenitors

Nucleic Acids Res. 2016 Oct 14;44(18):8610-8620. doi: 10.1093/nar/gkw765. Epub 2016 Aug 31.


Zika virus (ZIKV) infection causes microcephaly and has been linked to other brain abnormalities. How ZIKV impairs brain development and function is unclear. Here we systematically profiled transcriptomes of human neural progenitor cells exposed to Asian ZIKVC, African ZIKVM, and dengue virus (DENV). In contrast to the robust global transcriptome changes induced by DENV, ZIKV has a more selective and larger impact on expression of genes involved in DNA replication and repair. While overall expression profiles are similar, ZIKVC, but not ZIKVM, induces upregulation of viral response genes and TP53. P53 inhibitors can block the apoptosis induced by both ZIKVC and ZIKVM in hNPCs, with higher potency against ZIKVC-induced apoptosis. Our analyses reveal virus- and strain-specific molecular signatures associated with ZIKV infection. These datasets will help to investigate ZIKV-host interactions and identify neurovirulence determinants of ZIKV.

MeSH terms

  • Cell Death / genetics
  • Cell Line
  • Cerebral Cortex / cytology*
  • DNA Repair / genetics
  • DNA Replication / genetics
  • Dengue Virus / physiology
  • Gene Expression Profiling*
  • Humans
  • Neural Stem Cells / metabolism*
  • Neural Stem Cells / virology*
  • Signal Transduction / genetics
  • Species Specificity
  • Tumor Suppressor Protein p53 / metabolism
  • Up-Regulation / genetics
  • Zika Virus / physiology*
  • Zika Virus Infection / genetics*
  • Zika Virus Infection / virology


  • Tumor Suppressor Protein p53