Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes

Methods Mol Biol. 2016:1458:137-57. doi: 10.1007/978-1-4939-3801-8_11.


The adoptive transfer of a bolus of tumor-specific T lymphocytes into cancer patients is a promising therapeutic strategy. In one approach, tumor specificity is conferred upon T cells via engineering expression of exogenous receptors, such as chimeric antigen receptors (CARs). Here, we describe the generation and production of both murine and human CAR-engineered T lymphocytes using retroviruses.

Keywords: CAR-T cell; Chimeric antigen receptor; Gamma-retrovirus; Lentivirus; T lymphocytes.

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Gene Transfer Techniques
  • Genetic Engineering*
  • Genetic Vectors / genetics*
  • Humans
  • Immunotherapy, Adoptive
  • Lentivirus / genetics
  • Mice
  • Neoplasms / immunology
  • Neoplasms / therapy
  • Receptors, Antigen, T-Cell / genetics*
  • Receptors, Antigen, T-Cell / metabolism
  • Recombinant Fusion Proteins / genetics*
  • Recombinant Fusion Proteins / metabolism
  • Retroviridae / genetics
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism*
  • Transduction, Genetic


  • Receptors, Antigen, T-Cell
  • Recombinant Fusion Proteins