Single-Cell Cytokine Profiling to Investigate Cellular Functional Diversity in Hematopoietic Malignancies

Jonathan J Chen; Minsuk Kwak; Rong Fan

doi:10.1007/978-1-4939-4011-0_19

Single-Cell Cytokine Profiling to Investigate Cellular Functional Diversity in Hematopoietic Malignancies

Methods Mol Biol. 2016:1465:243-54. doi: 10.1007/978-1-4939-4011-0_19.

Authors

Jonathan J Chen¹, Minsuk Kwak¹, Rong Fan²

Affiliations

¹ Department of Biomedical Engineering, Yale University, PO Box 208260, New Haven, CT, 06520, USA.
² Department of Biomedical Engineering, Yale University, PO Box 208260, New Haven, CT, 06520, USA. rong.fan@yale.edu.

PMID: 27581152
DOI: 10.1007/978-1-4939-4011-0_19

Abstract

Single-cell analysis of cytokine production is increasingly recognized as an important method to understand the inflammatory microenvironment and hematopoietic disease state. Certain cytokines are critical to the regulation of lineage specification, and the aberrant production of these cytokines can contribute to lineage reprogramming. Here, we describe of a platform combining subnanoliter microchambers and a high-density antibody barcode array for the study of single-cell cytokine secretions in hematopoietic cancer cell populations.

Keywords: Cytokine secretions; Functional heterogeneity; High-throughput multiplexed analysis; Immunophenotyping; Pathogenesis; Single-cell cytokine profiling.

MeSH terms

Cytokines / metabolism*
Flow Cytometry
Hematologic Neoplasms / immunology*
Humans
Immunophenotyping
Single-Cell Analysis / methods*
Tissue Array Analysis

Substances

Cytokines