Transcriptional regulator PrqR plays a negative role in glucose metabolism and oxidative stress acclimation in Synechocystis sp. PCC 6803

Sci Rep. 2016 Sep 1;6:32507. doi: 10.1038/srep32507.

Abstract

Plant and cyanobacteria can perceive signals from soluble sugar and reactive oxygen species (ROS) and then coordinate gene expression under stress acclimation, but the underlying mechanism remains unclear. In this study, we found that the transcriptional factor PrqR (Slr0895) in Synechocystis can perceive signals from ROS generated after shifting from prolonged darkness with glucose into high-light. The deletion mutant (DprqR) showed increased growth rate and decreased ROS content, whereas the complementary strain (CprqR) restored the growth characteristics, phenotypes and ROS status of WT, thereby establishing PrqR as a negative regulator of ROS.LC/GC-MS-based metabolic profiling also showed active ROS mitigation in DprqR mutant. Further study by qRT-PCR, ChIP-PCR and deletion of both prqR and prqA (DprqR-DprqA mutant) revealed that PrqR exerts this negative regulation of ROS removal by controlling the expression of sodB and prqA (slr0896). Furthermore, PrqR also found to control glucose metabolism by regulating a positive regulator of glucose metabolism, sigE, and its regulons. Results suggest that PrqR was involved in perceiving signals from ROS under physiological condition, as well as in regulating stress removal and glucose metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological / genetics*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Gene Deletion
  • Gene Expression Regulation, Bacterial*
  • Genetic Complementation Test
  • Glucose / metabolism*
  • Metabolome
  • Oxidative Stress
  • Photoperiod
  • Reactive Oxygen Species / metabolism
  • Sigma Factor / genetics
  • Sigma Factor / metabolism
  • Superoxide Dismutase / genetics
  • Superoxide Dismutase / metabolism
  • Synechocystis / genetics*
  • Synechocystis / metabolism
  • Transcription Factors / deficiency
  • Transcription Factors / genetics*
  • Transcription, Genetic*

Substances

  • Bacterial Proteins
  • Reactive Oxygen Species
  • Sigma Factor
  • Transcription Factors
  • sigE protein, Bacteria
  • SodB protein, Bacteria
  • Superoxide Dismutase
  • Glucose