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. 2016 Sep 1;63(5):781-95.
doi: 10.1016/j.molcel.2016.08.021.

The Vici Syndrome Protein EPG5 Is a Rab7 Effector That Determines the Fusion Specificity of Autophagosomes With Late Endosomes/Lysosomes

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The Vici Syndrome Protein EPG5 Is a Rab7 Effector That Determines the Fusion Specificity of Autophagosomes With Late Endosomes/Lysosomes

Zheng Wang et al. Mol Cell. .

Abstract

Mutations in the human autophagy gene EPG5 cause the multisystem disorder Vici syndrome. Here we demonstrated that EPG5 is a Rab7 effector that determines the fusion specificity of autophagosomes with late endosomes/lysosomes. EPG5 is recruited to late endosomes/lysosomes by direct interaction with Rab7 and the late endosomal/lysosomal R-SNARE VAMP7/8. EPG5 also binds to LC3/LGG-1 (mammalian and C. elegans Atg8 homolog, respectively) and to assembled STX17-SNAP29 Qabc SNARE complexes on autophagosomes. EPG5 stabilizes and facilitates the assembly of STX17-SNAP29-VAMP7/8 trans-SNARE complexes, and promotes STX17-SNAP29-VAMP7-mediated fusion of reconstituted proteoliposomes. Loss of EPG5 activity causes abnormal fusion of autophagosomes with various endocytic vesicles, in part due to elevated assembly of STX17-SNAP25-VAMP8 complexes. SNAP25 knockdown partially suppresses the autophagy defect caused by EPG5 depletion. Our study reveals that EPG5 is a Rab7 effector involved in autophagosome maturation, providing insight into the molecular mechanism underlying Vici syndrome.

Keywords: LC3; RAB effector; SNARE; autophagosome maturation; epg-5.

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