Molecularly imprinted electrochemical sensor based on Au nanoparticles in carboxylated multi-walled carbon nanotubes for sensitive determination of olaquindox in food and feedstuffs

Biosens Bioelectron. 2017 Jan 15:87:417-421. doi: 10.1016/j.bios.2016.08.092. Epub 2016 Aug 28.


A novel molecularly imprinted electrochemical sensor (MIECS) was proposed to determine olaquindox (OLA) using gold nanoparticles in molecularly imprinted polymer (AuNPs@MIP) and carboxylated multi-walled carbon nanotubes (cMWCNTs). Glassy carbon electrode (GCE) was modified with cMWCNTs (cMWCNTs/GCE), and AuNPs/cMWCNT/GCE was obtained by electrodeposition on cMWCNTs/GCE using chronoamperometry in HAuCl4. Then, the obtained MIP/AuNPs/cMWCNTs/GCE was electropolymerized using OLA as template and o-PD as monomer to determine OLA. Important experimental parameters, namely, scan cycles, mole ratio of template molecules to functional monomers, pH value, and incubation time were optimized. The novel MIP sensor can offer a 2.7nM of detection limit for OLA. In addition, a series of food and feedstuffs were analyzed to demonstrate the feasibility of MIECS.

Keywords: Au nanoparticle; Carboxylated multi-walled carbon nanotube; Electrochemical determination; Molecularly imprinted electrochemical sensor; Olaquindox.

MeSH terms

  • Animal Feed / analysis
  • Animals
  • Biosensing Techniques / methods
  • Electrochemical Techniques / methods*
  • Fishes
  • Food Analysis / methods*
  • Gold / chemistry
  • Limit of Detection
  • Metal Nanoparticles / chemistry
  • Molecular Imprinting* / methods
  • Nanotubes, Carbon / chemistry
  • Nanotubes, Carbon / ultrastructure
  • Polymers / chemistry
  • Quinoxalines / analysis*
  • Red Meat / analysis
  • Seafood / analysis
  • Swine


  • Nanotubes, Carbon
  • Polymers
  • Quinoxalines
  • Gold
  • olaquindox