Electrochemiluminescence aptasensor of TiO2/CdS:Mn hybrids for ultrasensitive detection of cytochrome c

Na Bin; Weili Li; Xuehua Yin; Xiaohua Huang; Qingyun Cai

doi:10.1016/j.talanta.2016.07.046

Electrochemiluminescence aptasensor of TiO2/CdS:Mn hybrids for ultrasensitive detection of cytochrome c

Talanta. 2016 Nov 1:160:570-576. doi: 10.1016/j.talanta.2016.07.046. Epub 2016 Jul 25.

Authors

Na Bin¹, Weili Li¹, Xuehua Yin¹, Xiaohua Huang¹, Qingyun Cai²

Affiliations

¹ State Key Lab of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 China.
² State Key Lab of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082 China. Electronic address: qycai0001@hnu.edu.cn.

PMID: 27591652
DOI: 10.1016/j.talanta.2016.07.046

Abstract

A novel electrochemiluminescence (ECL) aptasensor was proposed for ultrasensitive detection of cytochrome c (cyt c) using CdS:Mn quantum dot-modified TiO2 nanowires (NWs) as electrode. The Mn-doped CdS was deposited on the TiO2 NWs by successive ion layer adsorption and reaction (SILAR) as ECL emitter, on which thiol-modified aptamer of cyt c was attached via Cd-S bond. Due to the high photo-electrical transfer efficiency, the as-prepared aptasensor shows high selectivity and sensitivity towards cyt c with a detection limit of 9.5fM and a linear range from 50fM to 125pM.

Keywords: CdS:Mn quantum dots; Cytochrome c; Electrochemiluminescence aptasensor; Titanium dioxide nanowires.

MeSH terms

Aptamers, Nucleotide / chemistry*
Biosensing Techniques
Cadmium Compounds / chemistry*
Cytochromes c / analysis*
Cytochromes c / chemistry
Electrochemical Techniques
Luminescent Measurements
Manganese / chemistry*
Nanowires / chemistry
Quantum Dots / chemistry*
Selenium Compounds / chemistry*
Titanium / chemistry*

Substances

Aptamers, Nucleotide
Cadmium Compounds
Selenium Compounds
titanium dioxide
Manganese
Cytochromes c
cadmium selenide
Titanium