A Panel of Embryonic Stem Cell Lines Reveals the Variety and Dynamic of Pluripotent States in Rabbits

Stem Cell Reports. 2016 Sep 13;7(3):383-398. doi: 10.1016/j.stemcr.2016.07.022. Epub 2016 Sep 1.


Conventional rabbit embryonic stem cell (ESC) lines are derived from the inner cell mass (ICM) of pre-implantation embryos using methods and culture conditions that are established for primate ESCs. In this study, we explored the capacity of the rabbit ICM to give rise to ESC lines using conditions similar to those utilized to generate naive ESCs in mice. On single-cell dissociation and culture in fibroblast growth factor 2 (FGF2)-free, serum-supplemented medium, rabbit ICMs gave rise to ESC lines lacking the DNA-damage checkpoint in the G1 phase like mouse ESCs, and with a pluripotency gene expression profile closer to the rabbit ICM/epiblast profiles. These cell lines can be converted to FGF2-dependent ESCs after culture in conventional conditions. They can also colonize the rabbit pre-implantation embryo. These results indicate that rabbit epiblast cells can be coaxed toward different types of pluripotent stem cells and reveal the dynamics of pluripotent states in rabbit ESCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers
  • Blastocyst / cytology
  • Blastocyst / metabolism
  • Cell Culture Techniques
  • Cell Cycle
  • Cell Differentiation* / genetics
  • Cell Line
  • Cell Self Renewal / genetics
  • Cells, Cultured
  • Computational Biology / methods
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / metabolism*
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Janus Kinases / metabolism
  • Leukemia Inhibitory Factor / metabolism
  • MicroRNAs / genetics
  • Rabbits
  • Signal Transduction
  • Transcriptome


  • Biomarkers
  • Leukemia Inhibitory Factor
  • MicroRNAs
  • Janus Kinases