Investigation of the binding of AuNPs-6-mercaptopurine and the sensitive detection of 6-mercaptopurine using resonance Rayleigh light scattering

Luminescence. 2017 Jun;32(4):502-508. doi: 10.1002/bio.3204. Epub 2016 Sep 4.

Abstract

A highly sensitive method for the detection of 6-mercaptopurine (MP) by resonance Rayleigh light scattering (RLS) method was developed. Gold nanoparticles (AuNPs) were synthesized by a modified seed method and characterized using transmission electron microscopy (TEM). AuNPs were bound to MP via covalent bonding to form the MP-AuNPs complex, which increased the RLS intensity of MP at 347 nm (increased by 65.7%). Under optimum conditions, the magnitude of the enhanced RLS intensity for MP-AuNPs was proportional to MP concentration in the range 0.0681-1.702 μg mL-1 . The linear regression equation was represented as follows: ΔIRLS = 9.31 + 82.42c (r = 0.9948). The limit of detection (LOD, 3σ) was 3.32 ng mL-1 . The system was applied successfully to detect MP in pharmaceuticals. MP recoveries were 99.9-101.7% with a relative standard deviation (RSD) (n = 5) of 0.59-0.77% for three synthetic samples, and 97.5-110.0% with an RSD of 0.98-2.10% (n = 5) for tablet samples.

Keywords: 6-mercaptopurine; assembly; gold nanoparticles; resonance Rayleigh light scattering.

MeSH terms

  • Calibration
  • Dynamic Light Scattering / methods*
  • Dynamic Light Scattering / standards
  • Gold / chemistry
  • Hydrogen-Ion Concentration
  • Limit of Detection
  • Linear Models
  • Mercaptopurine / analysis*
  • Mercaptopurine / chemistry
  • Mercaptopurine / metabolism
  • Metal Nanoparticles / chemistry*
  • Microscopy, Electron, Transmission
  • Osmolar Concentration
  • Sensitivity and Specificity
  • Spectrophotometry, Ultraviolet
  • Spectroscopy, Fourier Transform Infrared
  • Tablets / analysis

Substances

  • Tablets
  • Gold
  • Mercaptopurine