Platelet-rich plasma and hyaluronic acid - an efficient biostimulation method for face rejuvenation

J Cosmet Dermatol. 2017 Mar;16(1):112-119. doi: 10.1111/jocd.12271. Epub 2016 Sep 5.


Background: Cosmetic applications of platelet-rich plasma (PRP) are new, and reports are scarce and dispersed in the literature. There are a variety of commercially available kits and injection techniques, and the number and intervals of injections vary. New investigations should focus on developing a standardized procedure for PRP preparation and application methods to augment its efficacy and potency.

Objectives: In this report, we aim to provide data and commentary to assist and add to current guidelines.

Methods: A series of 94 female patients with varying degrees of facial aging signs were treated with PRP and hyaluronic acid (HA). Mean age was 53.0 ± 5.6. The mean injection number was 3.6 ± 2.0. Platelet-poor and platelet- rich plasma parts were mixed with 0.5 cc %3.5 hyaluronic acid and 0.5 cc procaine and injected with a 30G, 13-mm needle into deep dermis and hypodermis. Patients were asked to rate their personal satisfaction with their skin texture, pigmentation, and sagging. In addition, the overall results were rated by three independent physicians and the patients themselves. The outcomes were peer-reviewed, and correlations between the degree of the aesthetic scores and the number of injections were explored.

Results: There was a statistically significant difference in general appearance, skin firmness-sagging and skin texture according to the patients' before and after applications of PRP. A statistically significant correlation was found between the number of injections and overall satisfaction.

Conclusions: Compared to the baseline, the PRP and HA injections provided clinically visible and statistically significant improvement on facial skin. The improvements were more remarkable as the injection numbers increased.

Keywords: biostimulation; collagen; facial rejuvation; hyaluronic acid; injection; platelet-rich plasma (PRP) injection number.

MeSH terms

  • Cosmetic Techniques / adverse effects
  • Dermatologic Agents / administration & dosage
  • Dermatologic Agents / therapeutic use*
  • Face
  • Female
  • Humans
  • Hyaluronic Acid / administration & dosage
  • Hyaluronic Acid / therapeutic use*
  • Injections, Intradermal
  • Middle Aged
  • Patient Satisfaction
  • Platelet-Rich Plasma*
  • Practice Guidelines as Topic
  • Rejuvenation
  • Skin Aging*
  • Treatment Outcome


  • Dermatologic Agents
  • Hyaluronic Acid