A split-body, randomized, blinded study to evaluate the efficacy of a topical spray composed of essential oils and essential fatty acids from plant extracts with antimicrobial properties

Vet Dermatol. 2016 Dec;27(6):464-e123. doi: 10.1111/vde.12374. Epub 2016 Sep 5.


Background: Bacterial pyoderma is a frequent presentation in dogs. Despite the widespread availability of effective systemic and topical antimicrobial products, good clinical practice currently recommends avoidance of long-term use to mitigate the development of bacterial resistance.

Hypothesis/objectives: To evaluate the speed of resolution of clinical signs of bacterial pyoderma in dogs treated with a systemic antimicrobial agent with or without the use of an adjunctive spray with antimicrobial properties.

Animals: Twelve dogs with superficial bacterial pyoderma.

Methods: In this controlled and blinded study, all dogs were treated with oral cefalexin and a topical spray (PYOClean Spray) for 4 weeks. The spray was applied to one half of each dog's body, whereas a placebo spray was applied to the other half.

Results: Twelve dogs completed the study. Mean clinical scores were significantly reduced on spray-treated sites, for test product and placebo (respectively), by 47% and 34% at Week 1, 83% and 60% at Week 2, 95% and 82% at Week 3, and 100% and 96% at Week 4. Fifty percent of treated sites were considered clinically and cytologically cured at Week 2, 83% at Week 3, and 100% at Week 4 compared to 8%, 50% and 83% for the placebo sites, respectively.

Conclusions and clinical importance: These results demonstrate that use of a topical spray which contains plant-derived essential oils and fatty acids, and compounds with antimicrobial properties (Manuka oil and N-acetyl cysteine) may help to speed resolution of pyoderma and may allow for shorter antimicrobial treatment time.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Administration, Topical
  • Aerosols
  • Animals
  • Dog Diseases / drug therapy*
  • Dogs
  • Fatty Acids / administration & dosage
  • Fatty Acids / chemistry
  • Fatty Acids / therapeutic use*
  • Oils, Volatile / administration & dosage
  • Oils, Volatile / chemistry
  • Oils, Volatile / therapeutic use*
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Pyoderma / drug therapy
  • Pyoderma / veterinary*


  • Aerosols
  • Fatty Acids
  • Oils, Volatile
  • Plant Extracts