External quality assessment of absorbance measurements on spectral and spectral-line photometers

J Clin Chem Clin Biochem. 1989 May;27(5):323-9. doi: 10.1515/cclm.1989.27.5.323.

Abstract

From 1984-1987, 12 quality control surveys on photometric measurements were carried out in 600-800 laboratories. The participants measured the photometric absorbance of the control samples at 4 wavelengths of the mercury spectrum: 334.1 nm, 365.4 nm, 404.7 nm and 546.1 nm. The medians of the results were without exception lower than the target values, but only very few of them deviated more than 1%. The dispersion of the values did not follow a normal distribution. Two thirds of the values were concentrated within a very small range, while about 10% lay outside the 2- to 3-fold range. It was found that longer wavelengths resulted in a smaller dispersion of readings than shorter ones. Furthermore, precision showed a significant dependency on the absorbance readings of the samples, on the one hand, and on the different photometers, on the other.

MeSH terms

  • Chemistry, Clinical / standards*
  • Laboratories / standards*
  • Photometry / instrumentation*
  • Photometry / standards
  • Quality Control
  • Spectrophotometry / instrumentation*
  • Spectrophotometry / standards