Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Oct;363(19):fnw208.
doi: 10.1093/femsle/fnw208. Epub 2016 Sep 8.

Phage vB_BsuP-Goe1: The Smallest Identified Lytic Phage of Bacillus Subtilis

Affiliations

Phage vB_BsuP-Goe1: The Smallest Identified Lytic Phage of Bacillus Subtilis

Inka Marie Willms et al. FEMS Microbiol Lett. .

Abstract

Currently, interest in phage science is on the rise again as this subject remains largely unexplored, and its potential diversity mainly untapped. Here, we present phage vB_BsuP-Goe1, a new isolate that infects Bacillus subtilis The phage forms round plaques with a matt outline on agar plates containing a B. subtilis Δ6 lawn. Transmission electron microscopy and genomic analyses revealed that phage vB_BsuP-Goe1 belongs to the Podoviridae family as a new member of the Phi29likevirus genus. The phage genome comprises 18 379 bp that encode for one RNA gene and for 24 open reading frames. Phage vB_BsuP-Goe1 clusters with phage B103 and Nf within the Phi29likevirus genus and is currently the smallest member of this genus. Genome comparison with members of the Phi29likevirus genus reveals a conserved gene set coding for essential replication and morphogenesis functions while the genome extremities are more prone to gene and genome organisation variability.

Keywords: Bacillus subtilis; Goe1; Phi29likevirus; Picovirinae; Podoviridae; vB_BsuP-Goe1.

Similar articles

See all similar articles

Cited by 4 articles

Publication types

LinkOut - more resources

Feedback