Neuron-astrocyte interactions in spinal cord dorsal horn in neuropathic pain development and docosahexaenoic acid therapy

Igor V Manzhulo; Olga S Ogurtsova; Yuliya O Kipryushina; Nikolay A Latyshev; Sergey P Kasyanov; Inessa V Dyuizen; Anna A Tyrtyshnaia

doi:10.1016/j.jneuroim.2016.07.015

Neuron-astrocyte interactions in spinal cord dorsal horn in neuropathic pain development and docosahexaenoic acid therapy

J Neuroimmunol. 2016 Sep 15:298:90-7. doi: 10.1016/j.jneuroim.2016.07.015. Epub 2016 Jul 17.

Authors

Igor V Manzhulo¹, Olga S Ogurtsova², Yuliya O Kipryushina², Nikolay A Latyshev², Sergey P Kasyanov², Inessa V Dyuizen², Anna A Tyrtyshnaia³

Affiliations

¹ A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 17 Palchevskii Str., 690041, Russia; School of Biomedicine, Far Eastern Federal University, Vladivostok, 8 Sukhanova Str., 690950, Russia. Electronic address: i-manzhulo@bk.ru.
² A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 17 Palchevskii Str., 690041, Russia.
³ A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 17 Palchevskii Str., 690041, Russia; School of Biomedicine, Far Eastern Federal University, Vladivostok, 8 Sukhanova Str., 690950, Russia.

PMID: 27609281
DOI: 10.1016/j.jneuroim.2016.07.015

Abstract

The analgesic activity of docosahexaenoic acid (DHA, 22:6 n-3) was studied using a chronic constriction injury (CCI) rat model. Animals were subcutaneously injected with DHA emulsion at a dose of 4.5mg/kg (125mМ/kg) daily during 2weeks after surgery. We characterized the dynamics of GFAP-positive astrocyte, substance P (SP) and nNOS-positive neurons activity in the spinal cord dorsal horn (SCDH) superficial lamina. We found that DHA treatment decrease the intensity and duration of neurogenic pain syndrome, results in earlier stabilization of weight distribution, prevents the cold allodynia and dystrophic changings in denervated limb tissue. DHA treatment reduced the reactive astrocyte number, decrease SP-immunopositive fibers and nNOS-positive neurons number in the SCDH in neuropathic pain.

Keywords: Astrocyte; Chronic constriction injury; Docosahexaenoic acid; Nitric oxide synthase; Spinal cord dorsal horn; Substance P.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Analgesics / therapeutic use*
Analysis of Variance
Animals
Anti-Inflammatory Agents, Non-Steroidal / therapeutic use
Astrocytes / physiology*
Diclofenac / therapeutic use
Disease Models, Animal
Docosahexaenoic Acids / therapeutic use*
Glial Fibrillary Acidic Protein / metabolism
Hyperalgesia / diagnosis
Hyperalgesia / physiopathology
Male
Mice
Phosphopyruvate Hydratase / metabolism
Rats
Rats, Wistar
Sciatica / drug therapy*
Sciatica / pathology*
Sciatica / physiopathology
Spinal Cord Dorsal Horn / physiology*
Substance P / metabolism
Time Factors
Weight-Bearing / physiology*

Substances

Analgesics
Anti-Inflammatory Agents, Non-Steroidal
Glial Fibrillary Acidic Protein
Diclofenac
Docosahexaenoic Acids
Substance P
Phosphopyruvate Hydratase