The role of IL-1β and glutamate in the effects of lipopolysaccharide on the hippocampal electrical kindling of seizures

J Neuroimmunol. 2016 Sep 15;298:146-52. doi: 10.1016/j.jneuroim.2016.07.018. Epub 2016 Jul 21.


In our study, we used rapid electrical hippocampal kindling and in vivo microdialysis methods to assess the involvement of inflammatory mediators: lipopolysaccharide (LPS) and proinflammatory interleukin-1β (IL-1β) in mechanisms of epileptogenesis. We observed, that both, LPS and IL-1β, administered into stimulated hippocampus, accelerated kindling process. LPS also increased the expression of IL-1β in stimulated hippocampus in kindled rats. In vivo acute LPS perfusion, via a microdialysis cannula implanted into the naïve rat's hippocampus, produced an increase in extracellular glutamate release. We suppose, that particularly IL-1β action and increased glutamate concentration may significantly contribute to LPS effects on kindling development.

Keywords: Glutamate; IL-1β; LPS; Microdialysis; Rapid electrical hippocampal kindling; Seizures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Cytokines / metabolism
  • Disease Models, Animal
  • Electric Stimulation
  • Electroencephalography
  • Enzyme-Linked Immunosorbent Assay
  • Glutamic Acid / metabolism*
  • Hippocampus / drug effects*
  • Hippocampus / metabolism
  • Interleukin-1beta / metabolism*
  • Interleukin-1beta / pharmacology
  • Kindling, Neurologic / drug effects*
  • Lipopolysaccharides / toxicity*
  • Male
  • Microdialysis
  • Rats
  • Rats, Wistar
  • Seizures / chemically induced*
  • Time Factors
  • gamma-Aminobutyric Acid / metabolism


  • Cytokines
  • Interleukin-1beta
  • Lipopolysaccharides
  • Glutamic Acid
  • gamma-Aminobutyric Acid