Isoproterenol inhibits the increase in microvascular membrane permeability produced by bradykinin

J Trauma. 1989 Aug;29(8):1053-63; discussion 1063-4. doi: 10.1097/00005373-198908000-00002.


Bradykinin (BRADY) is hypothesized to cause the "capillary leak" syndrome in patients with sepsis, trauma, and burns. Our purpose was to determine if isoproterenol (ISO) reversed a BRADY-produced accelerated loss of intravascular fluid and protein into the interstitium of skin. An increase in microvascular permeability in canine hind paw skin was sustained by a continuous femoral artery infusion of BRADY (0.2 micrograms/kg/min). After 2 hours of BRADY, skin lymph flow (LYM FLOW microliters/min) increased nine-fold and skin lymph-to-plasma total protein concentration ratio (RTP) was substantially increased. Mean blood flow in the femoral arteries was increased four-fold by the BRADY infusion. After 2 hours of BRADY-induced increased permeability, five of the ten dogs were started on intravenous ISO (2 micrograms/min continuously) which increased heart rate from 182 +/- 15 to 222 +/- 11 beats/min. ISO reversed the increase in RTP produced by the BRADY. After 8 hours of BRADY, there was less tissue albumin in the dogs given ISO (14.5 +/- 2.0 vs. 29.5 +/- 6.6 mg/gram dry wgt, p less than 0.05 unpaired t-test). ISO can reverse the sustained increase in skin microvascular permeability produced by BRADY.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Blood Proteins / analysis
  • Bradykinin / pharmacology*
  • Cell Membrane Permeability / drug effects*
  • Dogs
  • Drug Interactions
  • Femoral Artery / drug effects
  • Femoral Artery / physiology
  • Infusions, Intra-Arterial
  • Infusions, Intravenous
  • Isoproterenol / pharmacology*
  • Lymph / analysis
  • Lymph / drug effects
  • Lymphatic System / drug effects*
  • Male
  • Regional Blood Flow / drug effects
  • Skin / blood supply
  • Skin / drug effects*
  • Time Factors


  • Blood Proteins
  • Isoproterenol
  • Bradykinin