Palatal wound healing using a xenogeneic collagen matrix - histological outcomes of a randomized controlled clinical trial

J Clin Periodontol. 2016 Dec;43(12):1124-1131. doi: 10.1111/jcpe.12624. Epub 2016 Oct 25.


Aim: The aim of this study was to test whether or not a collagen matrix can improve early wound healing compared to spontaneous healing based on histological and immunohistologic analyses.

Methods: In 20 volunteers, 6 mm punch biopsies were harvested at the palate. A xenogeneic collagen matrix (XCM) was sutured in one site; the other one was left untreated (control). Biopsies with a diameter of 8 mm were subsequently obtained at 4, 8, 15 and 29 days and histological and immunohistologic analyses were performed.

Results: At day 4, wound bed keratinization amounted to 12.4 ± 7.5% (control) and 18.0 ± 10.2% (XCM). This increased up to day 8 (19.7 ± 25.5% control; 29.1 ± 8.0% XCM) and reached complete keratinization at day 15 in both groups. The quantitative analyses of the superficial compartment measured an increase in the amount of granulation tissue (32-88% control; 14-41% XCM) from day 4 to day 8. Angiogenesis was first detected at 8 days. At day 29, the amount of connective tissue in all compartments reached values similar to the native tissue at baseline.

Conclusions: The application of a XCM as a wound dressing on palatal wounds might be beneficial in the early stages of wound healing. Further research with a larger sample size is needed to confirm these results.

Keywords: keratinized tissue; soft tissue augmentation; substitute; transplant (MeSH terms) collagen matrix; wound healing.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Collagen
  • Connective Tissue
  • Granulation Tissue
  • Humans
  • Palate
  • Wound Healing*


  • Collagen

Associated data

  • GENBANK/DM6000