Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice

Int J Biol Macromol. 2016 Dec;93(Pt A):904-912. doi: 10.1016/j.ijbiomac.2016.09.029. Epub 2016 Sep 9.


This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC). The GLPS3 was prepared from cultured mycelium and markedly alleviated the pancreatitis in mice through decreasing lipase, AMS, IFN-γ and TNF-α level as well as increasing SOD and total antioxidant activity. Furthermore, high throughput sequencing analysis revealed that GLPS3 altered the composition and diversity of intestinal microbiota, especially, decreased the relative abundance of phylum Bacteroidetes and increased that of phylum Firmictutes. At the genus level, supplementation of GLPS3 increased the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae. These results disclosed the potential therapy mechanism of GLPS3 on chronic pancreatitis might be intestinal microbiota dependent.

Keywords: Chronic pancreatitis; Ganoderma lucidum polysaccharide; High throughput sequencing; Intestinal microbiota.

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Antioxidants / therapeutic use
  • Bacteroidetes / genetics
  • Cytokines / metabolism
  • Drug Evaluation, Preclinical
  • Firmicutes / genetics
  • Fungal Polysaccharides / pharmacology*
  • Fungal Polysaccharides / therapeutic use
  • Gastrointestinal Microbiome / drug effects*
  • Mice, Inbred ICR
  • Molecular Typing
  • Pancreas / drug effects
  • Pancreas / enzymology
  • Pancreatitis, Chronic / drug therapy*
  • Proteobacteria / genetics
  • RNA, Ribosomal, 16S / genetics
  • Reishi / chemistry
  • Superoxide Dismutase / metabolism


  • Antioxidants
  • Cytokines
  • Fungal Polysaccharides
  • RNA, Ribosomal, 16S
  • Superoxide Dismutase