The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice

PLoS Genet. 2016 Sep 12;12(9):e1006311. doi: 10.1371/journal.pgen.1006311. eCollection 2016 Sep.

Abstract

Previous studies have shown that multivesicular bodies (MVBs)/endosomes-mediated vesicular trafficking may play key roles in plant immunity and cell death. However, the molecular regulation is poorly understood in rice. Here we report the identification and characterization of a MVBs-localized AAA ATPase LRD6-6 in rice. Disruption of LRD6-6 leads to enhanced immunity and cell death in rice. The ATPase activity and homo-dimerization of LRD6-6 is essential for its regulation on plant immunity and cell death. An ATPase inactive mutation (LRD6-6E315Q) leads to dominant-negative inhibition in plants. The LRD6-6 protein co-localizes with the MVBs marker protein RabF1/ARA6 and interacts with ESCRT-III components OsSNF7 and OsVPS2. Further analysis reveals that LRD6-6 is required for MVBs-mediated vesicular trafficking and inhibits the biosynthesis of antimicrobial compounds. Collectively, our study shows that the AAA ATPase LRD6-6 inhibits plant immunity and cell death most likely through modulating MVBs-mediated vesicular trafficking in rice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / biosynthesis*
  • Adenosine Triphosphatases / genetics
  • Arabidopsis / genetics
  • Arabidopsis / growth & development
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Cell Death / genetics
  • Disease Resistance / genetics
  • Disease Resistance / immunology
  • Endosomal Sorting Complexes Required for Transport / genetics
  • Endosomal Sorting Complexes Required for Transport / metabolism
  • Endosomes / genetics
  • Endosomes / metabolism
  • Gene Expression Regulation, Plant
  • Immunity, Cellular / genetics*
  • Multivesicular Bodies / genetics*
  • Multivesicular Bodies / immunology
  • Mutation
  • Oryza / genetics
  • Oryza / growth & development
  • Plant Diseases / genetics
  • Plant Diseases / immunology
  • Plant Leaves / genetics
  • Plant Leaves / immunology
  • Plant Proteins / genetics*
  • Plants, Genetically Modified / genetics*
  • Plants, Genetically Modified / immunology
  • Protein Transport / genetics
  • rab GTP-Binding Proteins / genetics

Substances

  • Arabidopsis Proteins
  • Endosomal Sorting Complexes Required for Transport
  • Plant Proteins
  • VPS2.1 protein, Arabidopsis
  • Adenosine Triphosphatases
  • Ara6 protein, Arabidopsis
  • rab GTP-Binding Proteins

Grant support

This work was supported by NSFC (Grant Numbers: 31171622 and 31571994), Transgenic Projects from the Chinese Ministry of Agriculture (TPCMA) (Grant Number: 2014ZX0800903B),) Program for New Century Excellent Talents in University from the Ministry of Education in China, “Hundred Talents Plan” Foundation, Youth foundation (Grant Number: 13QNJJ0076), and Youth Innovation Team Foundation (Grant Number: 14CXTD0039 from Sichuan, China) to XC; NSFC (Grant Number: 31401351) to JinW, (Grant Number: 31501627) to WL and (Grant Number: 31601290) to JY; Specialized Research Fund for doctoral program of higher education (Grant Number: 20135103120004) to JinW; Key Project of Sichuan Education Department (Grant Number: 15ZA0020 to WL; and TPCMA (Grant Number: 2016ZX08001002) to BM. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.