Patulibacter brassicae sp. nov., isolated from rhizosphere soil of Chinese cabbage (Brassica campestris)

Int J Syst Evol Microbiol. 2016 Dec;66(12):5056-5060. doi: 10.1099/ijsem.0.001469. Epub 2016 Sep 11.


A novel actinobacterial strain, designated SDT, was isolated from rhizosphere soil of Chinese cabbage in Shandong province, China. The cells were aerobic, Gram-staining-positive, oxidase- and catalase-positive, short rods and formed white colonies on trypticase soy agar. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and alanine, glutamic acid and leucine. Diphosphatidylglycerol was the predominant polar lipid. The predominant cellular fatty acid was C18 : 1ω9c; minor components were anteiso-C15 : 0 and anteiso-C17 : 0. The only isoprenoid quinone was demethylmenaquinone 7 (DMK-7), and the DNA G+C content was 72.7 mol%. Based on the full-length 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of strain SDT were Patulibacter medicamentivorans DSM 25962T (96.9 %), Patulibacter minatonensis DSM 18081T (96.7 %), Patulibacter americanus DSM 16676T (96.2 %) and Patulibacter ginsengiterrae DSM 25990T (95.9 %). Considering combined phenotypic and genotypic characteristics, it is proposed that strain SDT represents a novel species of the genus Patulibacter, for which the name Patulibacter brassicae sp. nov. is proposed. The type strain is SDT (=CICC 24108T=KCTC 39817T).

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Brassica / microbiology*
  • Cardiolipins / chemistry
  • DNA, Bacterial / genetics
  • Diaminopimelic Acid / chemistry
  • Fatty Acids / chemistry
  • Nucleic Acid Hybridization
  • Peptidoglycan / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Rhizosphere*
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry


  • Cardiolipins
  • DNA, Bacterial
  • Fatty Acids
  • Peptidoglycan
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • Diaminopimelic Acid
  • menaquinone 7