Isolation of human cDNA clones of ski and the ski-related gene, sno

Nucleic Acids Res. 1989 Jul 25;17(14):5489-500. doi: 10.1093/nar/17.14.5489.


cDNA clones of ski and the ski-related gene, sno, were obtained by screening human cDNA libraries. The predicted open reading frame of h-ski could encode a protein of 728 amino acid residues. The h-ski protein is highly homologous with the v-ski protein. The overall homology between h-ski and v-ski is 91% at the amino acid level. DNA sequencing analysis revealed two types of cDNA clones from the sno (ski-related novel gene) gene, possibly due to alternative splicing. The first type, named snoN (non Alu-containing), encoded a protein of 684 amino acid residues. The second type, named snoA (Alu-containing), encoded a protein of 415 amino acid residues. The first 366 amino acid residues of snoN and snoA are the same, but subsequent amino acids show divergence. Several transcripts of h-ski (6.0, 4.7, 3.8, 3.0, 2.1 and 1.8 kb) were detected. The mRNAs of h-sno were 6.2, 4.4 and 3.2kb.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution
  • Cell Line
  • DNA / genetics*
  • DNA / isolation & purification
  • DNA, Neoplasm / genetics*
  • DNA, Neoplasm / isolation & purification
  • DNA-Binding Proteins*
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Molecular Sequence Data
  • Multigene Family*
  • Oncogenes*
  • Proteins / genetics*
  • Proto-Oncogene Proteins*
  • Sequence Homology, Nucleic Acid
  • Software


  • DNA, Neoplasm
  • DNA-Binding Proteins
  • Intracellular Signaling Peptides and Proteins
  • Proteins
  • Proto-Oncogene Proteins
  • SKIL protein, human
  • SKI protein, human
  • DNA

Associated data

  • GENBANK/X15217
  • GENBANK/X15218
  • GENBANK/X15219