Synergistic Antipseudomonal Effects of Synthetic Peptide AMP38 and Carbapenems

Molecules. 2016 Sep 12;21(9):1223. doi: 10.3390/molecules21091223.


The aim was to explore the antimicrobial activity of a synthetic peptide (AMP38) and its synergy with imipenem against imipenem-resistant Pseudomonas aeruginosa. The main mechanism of imipenem resistance is the loss or alteration of protein OprD. Time-kill and minimal biofilm eradication concentration (MBEC) determinations were carried out by using clinical imipenem-resistant strains. AMP38 was markedly synergistic with imipenem when determined in imipenem-resistant P. aeruginosa. MBEC obtained for the combination of AMP38 and imipenem was of 62.5 μg/mL, whereas the MBEC of each antimicrobial separately was 500 μg/mL. AMP38 should be regarded as a promising antimicrobial to fight MDR P. aeruginosa infections. Moreover, killing effect and antibiofilm activity of AMP38 plus imipenem was much higher than that of colistin plus imipenem.

Keywords: Pseudomonas; antimicrobial peptides; biofilm eradication; synergism.

MeSH terms

  • Antimicrobial Cationic Peptides* / chemistry
  • Antimicrobial Cationic Peptides* / pharmacology
  • Biofilms / drug effects*
  • Biofilms / growth & development*
  • Carbapenems / pharmacology*
  • Pseudomonas aeruginosa / physiology*


  • Antimicrobial Cationic Peptides
  • Carbapenems