Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Dec;33(12):1685-1690.
doi: 10.1007/s10815-016-0811-4. Epub 2016 Sep 17.

Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection

Yamato Mizobe  1   2 , Naoto Oya  3 , Reiko Iwakiri  3 , Naomi Yoshida  3 , Yumi Sato  3 , Nanase Onoue  3 , Kazuchika Miyoshi  4 , Makoto Tokunaga  3 , Yuji Ezono  3
Affiliations
Free PMC article

Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection

Yamato Mizobe et al. J Assist Reprod Genet. 2016 Dec.
Free PMC article

Abstract

Purpose: In intracytoplasmic sperm injection (ICSI) of oocytes with a fragile oolemma (fragile oocytes), breakage can occur at injection. In this study, we produced embryos from oocytes with a fragile and normal oolemma (normal oocytes) by ICSI and compared their ability to be fertilized and develop in vitro. We also investigated whether fragile oocyte-derived embryos could implant after blastocyst transfer to determine whether fragile oocytes should be used for assisted reproductive technology treatment.

Methods: Oocytes were divided into three groups-normal oocytes from cycles containing no fragile oocytes (group A), normal oocytes from cycles containing at least one fragile oocyte (group B), and fragile oocytes (group C), and their fertilization abilities after ICSI and the developmental abilities of resultant embryos were compared.

Results: The fertilization rate in group C (65.3 %) was significantly (P < 0.01) lower than those in groups A (84.6 %) and B (86.9 %), and the degeneration rate in group C (24.2 %) was significantly (P < 0.01) higher than those in groups A (0.71 %) and B (0.28 %). However, there were no significant differences in the blastocyst formation rates (59.7-67.5 %) of embryos among the different groups. In addition, the pregnancy rate after transfer of blastocysts in group C (50.0 %) was not significantly different from those in groups A (35.6 %) and B (45.8 %).

Conclusions: The fertilization ability after ICSI of fragile oocytes is lower than that of normal oocytes but the resultant embryos have the same developmental ability as those of normal oocyte-derived embryos.

Keywords: Embryo; In vitro fertilization; Intracytoplasmic sperm injection; Oocyte plasma membrane; Oolemma; Time-lapse incubator.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflict of interest. Research involving human participants This study was approved by the Institutional Review Board of AIIKUKAI Medical Corporation. Informed consent Written informed consent for their treatment and for their outcomes to be described was obtained from all patients.

Figures

Fig. 1
Fig. 1
Fragile (a) and normal (b) oocytes. As shown in the circles, oolemma was broken by the insertion of an injection pipette in fragile oocytes. Scale bar 100 μm
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Dumoulin JM, Coonen E, Bras M, Bergers-Janssen JM, Ignoul-Vanvuchelen RC, van Wissen LC, et al. Embryo development and chromosomal anomalies after ICSI: effect of the injection procedure. Hum Reprod. 2001;16:306–12. doi: 10.1093/humrep/16.2.306. - DOI - PubMed
    1. Orief Y, Dafopoulos K, Al-Hassani S. Should ICSI be used in non-male factor infertility? Reprod BioMed Online. 2004;9:348–56. doi: 10.1016/S1472-6483(10)62152-9. - DOI - PubMed
    1. Palermo G, Joris H, Devroey P, Van Steirteghen AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;340:17–8. doi: 10.1016/0140-6736(92)92425-F. - DOI - PubMed
    1. Vanderzwalmen P, Bertin G, Lejeune B, Nijs M, Vandamme B, Schoysman R. Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolemma. Hum Reprod. 1996;11:540–7. doi: 10.1093/HUMREP/11.3.540. - DOI - PubMed
    1. Rosen MP, Shen S, Dobson AT, Fujimoto VY, McCulloch CE, Cedars MI. Oocyte degeneration after intracytoplasmic sperm injection: a multivariate analysis to assess its importance as a laboratory or clinical marker. Fertil Steril. 2006;85:1736–43. doi: 10.1016/j.fertnstert.2005.12.017. - DOI - PubMed

LinkOut - more resources