Effect of adenosine A1 analogue on tubuloglomerular feedback mechanism

Am J Physiol. 1989 Aug;257(2 Pt 2):F231-6. doi: 10.1152/ajprenal.1989.257.2.F231.


To evaluate further the role of adenosine in the transmission of tubuloglomerular feedback signals, we studied the effects of an adenosine receptor antagonist and an adenosine A1-receptor agonist on feedback-mediated changes in stop-flow pressure (SFP). In orthograde perfusion experiments conducted in anesthetized rats, systemic administration of the adenosine receptor blocker 1,3-dipropyl-8-sulfophenylxanthine (PSPX) did not inhibit feedback responses. Control SFP feedback responses averaged 9.7 +/- 0.65 before and 8.6 +/- 0.55 mmHg during systemic infusion of the receptor blocker. In retrograde perfusion experiments, intratubular administration of the A1 agonist (360 nM) N6-cyclopentyladenosine (CPA), added to a hypotonic solution, markedly enhanced feedback responses. This effect was completely prevented by coinfusion of PSPX. Addition of 10 mM of the antagonist to the CPA-containing solution attenuated SFP feedback responses to less than 1 mmHg (delta = 0.44 +/- 0.50). Furthermore, PSPX also inhibited feedback responses obtained with an isotonic solution alone. Furosemide, which has been shown to block normal SFP responses obtained with isotonic solutions, failed to block CPA-induced decreases in SFP. These data demonstrate that intraluminal administration of an adenosine A1 analogue causes feedback-mediated decreases in SFP and therefore support a role for adenosine receptors in the signal transmission pathway.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine / analogs & derivatives*
  • Adenosine / pharmacology
  • Animals
  • Feedback
  • Hypertonic Solutions
  • Hypotonic Solutions
  • Kidney Glomerulus / drug effects
  • Kidney Glomerulus / physiology*
  • Kidney Tubules / drug effects
  • Kidney Tubules / physiology*
  • Male
  • Perfusion
  • Rats
  • Rats, Inbred Strains
  • Receptors, Purinergic / drug effects
  • Receptors, Purinergic / physiology*
  • Xanthines / pharmacology*


  • Hypertonic Solutions
  • Hypotonic Solutions
  • Receptors, Purinergic
  • Xanthines
  • N(6)-cyclopentyladenosine
  • 1,3-dipropyl-8-(4-sulfophenyl)xanthine
  • Adenosine