Nationwide Surveillance of Novel Oxazolidinone Resistance Gene optrA in Enterococcus Isolates in China from 2004 to 2014

Lanqing Cui; Yang Wang; Yuan Lv; Shan Wang; Yunjia Song; Yun Li; Jian Liu; Feng Xue; Weiwei Yang; Jia Zhang

doi:10.1128/AAC.01256-16

Nationwide Surveillance of Novel Oxazolidinone Resistance Gene optrA in Enterococcus Isolates in China from 2004 to 2014

Antimicrob Agents Chemother. 2016 Nov 21;60(12):7490-7493. doi: 10.1128/AAC.01256-16. Print 2016 Dec.

Authors

Lanqing Cui¹, Yang Wang², Yuan Lv³, Shan Wang¹, Yunjia Song¹, Yun Li¹, Jian Liu¹, Feng Xue¹, Weiwei Yang¹, Jia Zhang¹

Affiliations

¹ Institute of Clinical Pharmacology, Peking University First Hospital, Beijing, China.
² Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China.
³ Institute of Clinical Pharmacology, Peking University First Hospital, Beijing, China lvyuan0901@sina.com.

Abstract

A total of 2,201 nonduplicate enterococcal isolates collected from 29 hospitals in 23 cities in China between 2004 and 2014 were screened for the oxazolidinone resistance gene optrA; 45 isolates (2.0%) were optrA positive with 11 OptrA variants identified. The positive rate of optrA increased from 0.4% to 3.9% during the 10-year surveillance period. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence type (MLST) analysis revealed that 37 optrA-positive Enterococcus faecalis isolates clustered into 25 PFGE patterns and 21 sequence types, while 6 Enterococcus faecium isolates represented 6 PFGE patterns and 6 sequence types. The present study underscores the importance of routine and persistent monitoring of oxazolidinone resistance and optrA gene.

Publication types

Multicenter Study
Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
China / epidemiology
Drug Resistance, Bacterial / genetics*
Electrophoresis, Gel, Pulsed-Field
Enterococcus faecalis / drug effects
Enterococcus faecalis / genetics*
Enterococcus faecalis / growth & development
Enterococcus faecalis / isolation & purification
Enterococcus faecium / drug effects
Enterococcus faecium / genetics*
Enterococcus faecium / growth & development
Enterococcus faecium / isolation & purification
Genes, Bacterial*
Genotype
Gram-Positive Bacterial Infections / drug therapy
Gram-Positive Bacterial Infections / epidemiology*
Gram-Positive Bacterial Infections / microbiology
Humans
Microbial Sensitivity Tests
Multilocus Sequence Typing
Mutation
Oxazolidinones / pharmacology
Public Health Surveillance

Substances

Anti-Bacterial Agents
Oxazolidinones

Grants and funding

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.