Development and evaluation of a multiplex real-time PCR for the detection of IMP, VIM, and OXA-23 carbapenemase gene families on the BD MAX open system

Diagn Microbiol Infect Dis. 2016 Dec;86(4):358-361. doi: 10.1016/j.diagmicrobio.2016.08.019. Epub 2016 Aug 26.

Abstract

A multiplex real-time polymerase chain reaction (PCR) assay was developed for the detection of clinically prevalent IMP, VIM, and OXA-23 gene families. The assay was designed to work on the BD MAX open platform which is a fully automated system for all PCR processes including sample extraction to PCR resulting. A total of 107 well-characterized carbapenem resistant Enterobacteriaceae were evaluated and the results were 100% concordant with the reference test isolates. This assay will serve to complement PCR screens that detect the major carbapenemase families of NDM, KPC, and OXA-48-like.

Keywords: Carbapenem resistance; Enterobacteriaceae; Real-time PCR.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Proteins / analysis*
  • Bacterial Proteins / genetics*
  • Bacteriological Techniques / methods
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / genetics
  • Enterobacteriaceae Infections / microbiology
  • Humans
  • Multiplex Polymerase Chain Reaction / methods*
  • Real-Time Polymerase Chain Reaction / methods*
  • beta-Lactamases / analysis*
  • beta-Lactamases / genetics*

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase