Micellar Electrokinetic Chromatography of Aminoglycosides

Methods Mol Biol. 2016:1483:77-90. doi: 10.1007/978-1-4939-6403-1_5.

Abstract

The components of the aminoglycosides, e.g., gentamicin, sisomicin, netilmicin, kanamycin, amikacin, and tobramycin, and related impurities of these antibiotics can be separated by means of micellar electrokinetic chromatography (MEKC). Derivatization with o-phthaldialdehyde and thioglycolic acid is found to be appropriate for these antibiotics. The background electrolyte was composed of sodium tetraborate (100 mM), sodium deoxycholate (20 mM), and β-cyclodextrin (15 mM) having a pH value of 10.0. This method is valid for evaluation of gentamicin, kanamycin, and tobramycin. It has to be adopted for amikacin, paromomycin, neomycin, and netilmicin.

Keywords: Aminoglycosides; Background electrolyte; Bile salts; Cyclodextrins; Gentamicin; Kanamycin; MEKC; Tobramycin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amikacin / chemistry
  • Amikacin / isolation & purification
  • Aminoglycosides / chemistry
  • Aminoglycosides / isolation & purification*
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / isolation & purification*
  • Chromatography, Micellar Electrokinetic Capillary / methods*
  • Kanamycin / chemistry
  • Kanamycin / isolation & purification
  • Micelles
  • Netilmicin / chemistry
  • Netilmicin / isolation & purification
  • Sisomicin / chemistry
  • Sisomicin / isolation & purification
  • Tobramycin / chemistry
  • Tobramycin / isolation & purification
  • beta-Cyclodextrins / chemistry
  • beta-Cyclodextrins / isolation & purification

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • Micelles
  • beta-Cyclodextrins
  • Netilmicin
  • Kanamycin
  • Amikacin
  • Tobramycin
  • Sisomicin