Pyrosequencing Analysis of Norovirus Genogroup II Distribution in Sewage and Oysters: First Detection of GII.17 Kawasaki 2014 in Oysters

Jian Pu; Shinobu Kazama; Takayuki Miura; Nabila Dhyan Azraini; Yoshimitsu Konta; Hiroaki Ito; You Ueki; Ermaya Eka Cahyaningrum; Tatsuo Omura; Toru Watanabe

doi:10.1007/s12560-016-9261-5

Pyrosequencing Analysis of Norovirus Genogroup II Distribution in Sewage and Oysters: First Detection of GII.17 Kawasaki 2014 in Oysters

Food Environ Virol. 2016 Dec;8(4):310-312. doi: 10.1007/s12560-016-9261-5. Epub 2016 Sep 19.

Authors

Affiliations

¹ Department of Food, Life and Environmental Sciences, Yamagata University, 1-23 Wakaba-machi, Tsuruoka, Yamagata, 997-8555, Japan. pu@tds1.tr.yamagata-u.ac.jp.
² New Industry Creation Hatchery Center, Tohoku University, 6-6-04 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8579, Japan.
³ Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta, Indonesia.
⁴ Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto, Kumamoto, 860-8555, Japan.
⁵ Miyagi Prefectural Institute of Public Health and Environment, 4-7-2, Saiwaicho, Miyagino-ku, Sendai, Miyagi, 983-8666, Japan.
⁶ Department of Food, Life and Environmental Sciences, Yamagata University, 1-23 Wakaba-machi, Tsuruoka, Yamagata, 997-8555, Japan.

PMID: 27646397
DOI: 10.1007/s12560-016-9261-5

Abstract

Norovirus GII.3, GII.4, and GII.17 were detected using pyrosequencing in sewage and oysters in January and February 2015, in Japan. The strains in sewage and oyster samples were genetically identical or similar, predominant strains belonging to GII.17 Kawasaki 2014 lineage. This is the first report of GII.17 Kawasaki 2014 in oysters.

Keywords: Norovirus; Oyster; Pyrosequencing; Sewage.

MeSH terms

Animals
Food Contamination / analysis*
Genotype
Humans
Norovirus / classification
Norovirus / genetics
Norovirus / isolation & purification*
Ostreidae / virology*
Phylogeny
Sewage / virology*
Shellfish / virology*

Substances

Sewage