Agaricus blazei extract attenuates rotenone-induced apoptosis through its mitochondrial protective and antioxidant properties in SH-SY5Y neuroblastoma cells

Nutr Neurosci. 2018 Feb;21(2):97-107. doi: 10.1080/1028415X.2016.1222332. Epub 2016 Sep 20.

Abstract

The present study was aimed to find out the effect of Agaricus blazei mushroom extract against rotenone-induced cellular model. SH-SY5Y neuroblastoma cells are divided into four experimental groups (control, rotenone (100 nM), A. blazei (5 μg/ml) + rotenone (100 nM), and A. blazei alone treated) based on MTT assay, cells were allowed to measure the ROS, TBARS levels, and antioxidants activities. Finally, mitochondrial transmembrane potential (MMP) and expressions of apoptotic proteins were also analyzed. Pre-treatment with A. blazei significantly enhanced cell viability, attenuated rotenone-induced ROS, MMP, and apoptosis. Our results indicated that anti-apoptotic properties of this natural compound due to its antioxidant and mitochondrial protective function protect rotenone-induced cytotoxicity. Therefore, it may be concluded that A. blazei can be further developed as a promising drug for the treatment of Parkinson's disease (PD).

Keywords: A. blazei; Apoptosis; Mitochondria membrane potential; Oxidative stress; Rotenone.

MeSH terms

  • Agaricales / chemistry
  • Agaricus / chemistry*
  • Antioxidants / pharmacology*
  • Apoptosis / drug effects*
  • Apoptosis Regulatory Proteins / genetics
  • Apoptosis Regulatory Proteins / metabolism
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Glutathione / metabolism
  • Humans
  • Membrane Potential, Mitochondrial / drug effects
  • Mitochondria / drug effects*
  • Mitochondria / metabolism
  • Neuroblastoma / drug therapy
  • Oxidative Stress / drug effects
  • Parkinson Disease
  • Reactive Oxygen Species / metabolism
  • Rotenone / toxicity*
  • Superoxide Dismutase / metabolism
  • Thiobarbituric Acid Reactive Substances / metabolism

Substances

  • Antioxidants
  • Apoptosis Regulatory Proteins
  • Reactive Oxygen Species
  • Thiobarbituric Acid Reactive Substances
  • Rotenone
  • Superoxide Dismutase
  • Glutathione