Molecular cloning and sequence analysis of cDNA coding for canine gastrin

Biochem Int. 1989 Mar;18(3):631-6.

Abstract

We have isolated and sequenced cDNA clones encoding for preprogastrin from a canine antral cDNA library. Comparison of this sequence with those of porcine, human, and rat gastrin reveals extensive (83%) homology in the gastrin coding region as well as short regions of conserved nucleotides in the noncoding regions. The canine sequence encodes a preprogastrin of 104 amino acids which consists of a signal peptide, a 37-amino acid prosegment, and a 34-amino acid gastrin sequence, followed by a glycine (the amide donor), and flanked by pairs of arginine residues.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Blotting, Northern
  • Cloning, Molecular*
  • DNA / analysis*
  • Dogs
  • Gastrins / analysis
  • Gastrins / genetics*
  • Genetic Code
  • Molecular Probes
  • Molecular Sequence Data
  • Protein Precursors / genetics*
  • Protein Sorting Signals / analysis
  • RNA, Messenger / analysis*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • Amino Acids
  • Gastrins
  • Molecular Probes
  • Protein Precursors
  • Protein Sorting Signals
  • RNA, Messenger
  • preprogastrin
  • DNA