Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Sep 20;17:387.
doi: 10.1186/s12859-016-1242-z.

DBSecSys 2.0: A Database of Burkholderia Mallei and Burkholderia Pseudomallei Secretion Systems

Affiliations
Free PMC article

DBSecSys 2.0: A Database of Burkholderia Mallei and Burkholderia Pseudomallei Secretion Systems

Vesna Memišević et al. BMC Bioinformatics. .
Free PMC article

Abstract

Background: Burkholderia mallei and B. pseudomallei are the causative agents of glanders and melioidosis, respectively, diseases with high morbidity and mortality rates. B. mallei and B. pseudomallei are closely related genetically; B. mallei evolved from an ancestral strain of B. pseudomallei by genome reduction and adaptation to an obligate intracellular lifestyle. Although these two bacteria cause different diseases, they share multiple virulence factors, including bacterial secretion systems, which represent key components of bacterial pathogenicity. Despite recent progress, the secretion system proteins for B. mallei and B. pseudomallei, their pathogenic mechanisms of action, and host factors are not well characterized.

Results: We previously developed a manually curated database, DBSecSys, of bacterial secretion system proteins for B. mallei. Here, we report an expansion of the database with corresponding information about B. pseudomallei. DBSecSys 2.0 contains comprehensive literature-based and computationally derived information about B. mallei ATCC 23344 and literature-based and computationally derived information about B. pseudomallei K96243. The database contains updated information for 163 B. mallei proteins from the previous database and 61 additional B. mallei proteins, and new information for 281 B. pseudomallei proteins associated with 5 secretion systems, their 1,633 human- and murine-interacting targets, and 2,400 host-B. mallei interactions and 2,286 host-B. pseudomallei interactions. The database also includes information about 13 pathogenic mechanisms of action for B. mallei and B. pseudomallei secretion system proteins inferred from the available literature or computationally. Additionally, DBSecSys 2.0 provides details about 82 virulence attenuation experiments for 52 B. mallei secretion system proteins and 98 virulence attenuation experiments for 61 B. pseudomallei secretion system proteins. We updated the Web interface and data access layer to speed-up users' search of detailed information for orthologous proteins related to secretion systems of the two pathogens.

Conclusions: The updates of DBSecSys 2.0 provide unique capabilities to access comprehensive information about secretion systems of B. mallei and B. pseudomallei. They enable studies and comparisons of corresponding proteins of these two closely related pathogens and their host-interacting partners. The database is available at http://dbsecsys.bhsai.org .

Keywords: Bacterial secretion system; Burkholderia mallei; Burkholderia pseudomallei; Host-pathogen interactions; Pathogenic mechanisms of action; Virulence factors.

Figures

Fig. 1
Fig. 1
Comparison of related secretion systems in Burkholderia mallei and B. pseudomallei. The genome browser shows the location of the orthologous proteins TssM, TssN, TssF, and TssG and their association with related secretion systems on the bacterial reference sequences for B. mallei strain ATCC 23344 and B. pseudomallei strain K96243. Type 2 secretion system (T2SS) and type 6 secretion system (T6SS) proteins are depicted in red and yellow, respectively

Similar articles

See all similar articles

Cited by 2 articles

References

    1. Losada L, Ronning CM, DeShazer D, Woods D, Fedorova N, Kim HS, Shabalina SA, Pearson TR, Brinkac L, Tan P, et al. Continuing evolution of Burkholderia mallei through genome reduction and large-scale rearrangements. Genome Biol Evol. 2010;2:102–116. doi: 10.1093/gbe/evq003. - DOI - PMC - PubMed
    1. Allwood EM, Devenish RJ, Prescott M, Adler B, Boyce JD. Strategies for intracellular survival of Burkholderia pseudomallei. Front Microbiol. 2011;2:170. doi: 10.3389/fmicb.2011.00170. - DOI - PMC - PubMed
    1. Willcocks SJ, Denman CC, Atkins HS, Wren BW. Intracellular replication of the well-armed pathogen Burkholderia pseudomallei. Curr Opin Microbiol. 2016;29:94–103. doi: 10.1016/j.mib.2015.11.007. - DOI - PubMed
    1. Hatcher CL, Muruato LA, Torres AG. Recent Advances in Burkholderia mallei and B. pseudomallei Research. Curr Trop Med Rep. 2015;2(2):62–69. doi: 10.1007/s40475-015-0042-2. - DOI - PMC - PubMed
    1. Stone JK, DeShazer D, Brett PJ, Burtnick MN. Melioidosis: molecular aspects of pathogenesis. Expert Rev Anti Infect Ther. 2014;12(12):1487–1499. doi: 10.1586/14787210.2014.970634. - DOI - PMC - PubMed

MeSH terms

LinkOut - more resources

Feedback