Complete Genome Sequence of the Intracellular Bacterial Symbiont TC1 in the Anaerobic Ciliate Trimyema compressum

Naoya Shinzato; Hiroaki Aoyama; Seikoh Saitoh; Naruo Nikoh; Kazuma Nakano; Makiko Shimoji; Misuzu Shinzato; Kazuhito Satou; Kuniko Teruya; Takashi Hirano; Takanori Yamada; Masaru K Nobu; Hideyuki Tamaki; Yumi Shirai; Sanghwa Park; Takashi Narihiro; Wen-Tso Liu; Yoichi Kamagata

doi:10.1128/genomeA.01032-16

Complete Genome Sequence of the Intracellular Bacterial Symbiont TC1 in the Anaerobic Ciliate Trimyema compressum

Genome Announc. 2016 Sep 22;4(5):e01032-16. doi: 10.1128/genomeA.01032-16.

Authors

Naoya Shinzato¹, Hiroaki Aoyama², Seikoh Saitoh³, Naruo Nikoh⁴, Kazuma Nakano⁵, Makiko Shimoji⁵, Misuzu Shinzato⁵, Kazuhito Satou⁵, Kuniko Teruya⁵, Takashi Hirano⁵, Takanori Yamada³, Masaru K Nobu⁶, Hideyuki Tamaki⁷, Yumi Shirai³, Sanghwa Park³, Takashi Narihiro⁷, Wen-Tso Liu⁸, Yoichi Kamagata⁷

Affiliations

¹ Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa, Japan Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan naoya-s@comb.u-ryukyu.ac.jp.
² Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa, Japan Center for Strategic Research Project, University of the Ryukyus, Nishihara, Okinawa, Japan.
³ Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa, Japan.
⁴ Department of Liberal Arts, The Open University of Japan, Chiba, Chiba, Japan.
⁵ Okinawa Institute of Advanced Sciences, Uruma, Okinawa, Japan.
⁶ Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
⁷ Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
⁸ Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

Abstract

A free-living ciliate, Trimyema compressum, found in anoxic freshwater environments harbors methanogenic archaea and a bacterial symbiont named TC1 in its cytoplasm. Here, we report the complete genome sequence of the TC1 symbiont, consisting of a 1.59-Mb chromosome and a 35.8-kb plasmid, which was determined using the PacBio RSII sequencer.