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. 2017;1468:63-89.
doi: 10.1007/978-1-4939-4035-6_7.

Deciphering Noncoding RNA and Chromatin Interactions: Multiplex Chromatin Interaction Analysis by Paired-End Tag Sequencing (mChIA-PET)

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Deciphering Noncoding RNA and Chromatin Interactions: Multiplex Chromatin Interaction Analysis by Paired-End Tag Sequencing (mChIA-PET)

Jocelyn Choy et al. Methods Mol Biol. .

Abstract

Genomic DNA is dynamically associated with protein factors and folded to form chromatin fibers. The 3-dimensional (3D) configuration of the chromatin will enable the distal genetic elements to come into close proximity, allowing transcriptional regulation. Noncoding RNA can mediate the 3D structure of chromatin. Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET) is a valuable and powerful technique in molecular biology which allows the study of unbiased, genome-wide de novo chromatin interactions with paired-end tags. Here, we describe the standard version of ChIA-PET and a Multiplex ChIA-PET version.

Keywords: Antibodies; Barcoded half-linkers; Chromatin immunoprecipitation (ChIP); Chromatin interactions; High-throughput sequencing; Multiplex ChIA-PET; Paired-end tags; Protein; Proximity ligation.

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