Axonal protection by thioredoxin-1 with inhibition of interleukin-1β in TNF-induced optic nerve degeneration

Exp Eye Res. 2016 Nov;152:71-76. doi: 10.1016/j.exer.2016.09.007. Epub 2016 Sep 21.


Interleukin (IL)-1β, a proinflammatory cytokine, is a key mediator in several acute and chronic neurological diseases. Thioredoxin-1 (TRX1) acts as an antioxidant and plays a protective role in certain neurons. We examined whether exogenous TRX1 exerts axonal protection and affects IL-1β levels in tumor necrosis factor (TNF)-induced optic nerve degeneration in rats. Immunoblot analysis showed that IL-1β was upregulated in the optic nerve after intravitreal injection of TNF. Treatment with recombinant human (rh) TRX1 exerted substantial protective effects against TNF-induced axonal loss. The increase in the IL-1β level in the optic nerve was abolished by rhTRX1. Treatment with rhTRX1 also significantly inhibited increased glial fibrillary acidic protein (GFAP) levels induced by TNF. Immunohistochemical analysis showed substantial colocalization of IL-1β and GFAP in the optic nerve after TNF injection. These results suggest that IL-1β is upregulated in astrocytes in the optic nerve after TNF injection and that exogenous rhTRX1 exerts axonal protection with an inhibitory effect on IL-1β.

Keywords: Astrocyte; Interleukin-1β; Optic nerve; Thioredoxin; Tumor necrosis factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axons / drug effects
  • Axons / metabolism
  • Axons / pathology
  • Blotting, Western
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Interleukin-1beta / antagonists & inhibitors*
  • Interleukin-1beta / metabolism
  • Intravitreal Injections
  • Male
  • Nerve Degeneration / metabolism
  • Nerve Degeneration / pathology
  • Nerve Degeneration / prevention & control*
  • Optic Nerve / drug effects
  • Optic Nerve / pathology*
  • Optic Nerve Diseases / metabolism
  • Optic Nerve Diseases / pathology
  • Optic Nerve Diseases / prevention & control*
  • Rats
  • Recombinant Proteins / administration & dosage*
  • Thioredoxins / administration & dosage*
  • Tumor Necrosis Factor-alpha / toxicity


  • Interleukin-1beta
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Txn1 protein, rat
  • Thioredoxins