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. 2016;1490:15-34.
doi: 10.1007/978-1-4939-6433-8_2.

Secondary Structure Prediction of Single Sequences Using RNAstructure

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Secondary Structure Prediction of Single Sequences Using RNAstructure

Zhenjiang Zech Xu et al. Methods Mol Biol. .

Abstract

RNA secondary structure is often predicted using folding thermodynamics. RNAstructure is a software package that includes structure prediction by free energy minimization, prediction of base pairing probabilities, prediction of structures composed of highly probably base pairs, and prediction of structures with pseudoknots. A user-friendly graphical user interface is provided, and this interface works on Windows, Apple OS X, and Linux. This chapter provides protocols for using RNAstructure for structure prediction.

Keywords: RNA folding thermodynamics; RNA statistical mechanics; RNA structure prediction.

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