The prevalence of autoantibody and its relationship with genotypes of hepatitis C virus in patients with chronic hepatitis C virus infection

APMIS. 2016 Nov;124(11):979-984. doi: 10.1111/apm.12593. Epub 2016 Sep 27.


The prevalence of autoantibody in the patients with chronic hepatitis C infection, and the relationship between the autoantibodies and HCV genotypes were investigated in this study. One hundred and eight anti-HCV positive and 86 anti-HCV negative patients were included in the study. Anti-HCV were studied by enzyme immunassay (EIA). HCV RNA was determined by real time polymerase chain reaction (PCR) and HCV genotypes were determined by a reverse-line blot hybridization. Anti-nuclear antibodies (ANA), anti-smooth muscle antibodies (ASMA), Anti-mitochondrial antibodies (AMA), liver kidney microsomal antibodies (LKM) were detected by indirect immunofluorescence assay. Among patients, 13 (12.03%) of 108 were positive for at least one autoantibody. The positivity was not observed in control group. The most prevalent autoantibody in anti-HCV positive group was ANA. ANA was positive in six HCV patients with genotype 1. In HCV patients with genotype 1, the frequencies of ANA, ASMA, AMA and LKM1 were six, two, three and one, respectively. In HCV patients with genotype 2, ANA was positive one patient and ASMA, AMA and LKM1 were not detected in HCV patients with genotype 2. In conclusion, the autoantibodies in patients with chronic hepatitis C in the study were low as compared to those reported in previous studies.

Keywords: Hepatitis C virus; anti-mitochondrial antibodies; anti-nuclear antibodies; anti-smooth muscle antibodies; autoantibody; liver kidney microsomal antibodies.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Autoantibodies / blood*
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Genotype*
  • Genotyping Techniques
  • Hepacivirus / classification*
  • Hepacivirus / genetics
  • Hepacivirus / isolation & purification
  • Hepatitis C Antibodies / blood
  • Hepatitis C, Chronic / pathology*
  • Hepatitis C, Chronic / virology*
  • Humans
  • Immunoenzyme Techniques
  • Male
  • Middle Aged
  • Nucleic Acid Hybridization
  • Prevalence
  • RNA, Viral / blood
  • Real-Time Polymerase Chain Reaction
  • Young Adult


  • Autoantibodies
  • Hepatitis C Antibodies
  • RNA, Viral