Objective: To evaluate the ability of casein phosphopeptide/amorphous calcium phosphate (CPP/ACP) and lysozyme, lactoferrin, and lactoperoxidase (LLL) added to glass ionomer cement (GIC) to inhibit the growth of S. mutans in a caries model.
Material and methods: Eighty permanent third molars were selected. The dentin of these teeth was exposed and flattened. Except for the coronal dentin, the specimens were waterproofed, autoclaved, and submitted to cariogenic challenge with standard strain of S. mutans. The carious lesions were sealed as follows: group 1 (n=20): GIC without additives; group 2 (n=20): GIC + CPP/ACP; group 3 (n=20): GIC + LLL; group 4 (n=20): GIC + CPP/ACP + LLL. S. mutans counts were performed before the caries were sealed (n=5), after 24 hours (n=5), at 1 month (n=5), and at 6 months (n=5). The results were analyzed using descriptive statistical analysis and the Kruskal-Wallis test (Student-Newman-Keuls test).
Results: GIC + LLL caused a significant reduction of S. mutans 1 month after sealing (p<0.01); however, there was a significant growth of S. mutans 6 months after sealing. GIC, GIC + CPP/ACP, and GIC + CPP/ACP + LLL showed similar behavior with significant reduction of S. mutans after 24 hours (p<0.05) and increase after 1 and 6 months.
Conclusion: The addition of LLL to GIC increases the antimicrobial action of GIC on S. mutans. This leads to control of bacterial biofilm for 1 month, thus stopping the progression of carious lesions.
Keywords: Anti-Bacterial Agents; Caries; Glass Ionomer Cements; Lactoferrin; Lactoperoxidase; Muramidase; Streptococcus mutans; casein phosphopeptide-amorphous calcium phosphate nanocomplex.
Effectiveness of Casein Phosphopeptide-Amorphous Calcium Phosphate and Lysozyme, Lactoferrin, and Lactoperoxidase in Reducing Streptococcus Mutans Counts in Dentinal CariesSL Pinheiro et al. Gen Dent 65 (2), 47-50. PMID 28253182.This study compared the capacity of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) to that of a combination of lysozyme, lactoferrin, and lactoperoxidase (LL …
Casein Phosphopeptide-Amorphous Calcium Phosphate Reduces Streptococcus Mutans Biofilm Development on Glass Ionomer Cement and Disrupts Established BiofilmsSG Dashper et al. PLoS One 11 (9), e0162322. PMID 27589264.Glass ionomer cements (GIC) are dental restorative materials that are suitable for modification to help prevent dental plaque (biofilm) formation. The aim of this study w …
Prevention of Secondary Caries Using Silver Diamine Fluoride Treatment and Casein Phosphopeptide-Amorphous Calcium Phosphate Modified Glass-Ionomer CementIS Zhao et al. J Dent 57, 38-44. PMID 27939657.SDF treatment and incorporation of CPP-ACP into GIC restorative material can prevent secondary root caries development.
Anticariogenicity of Casein Phosphopeptide-Amorphous Calcium Phosphate: A Review of the LiteratureC Llena et al. J Contemp Dent Pract 10 (3), 1-9. PMID 19430620. - ReviewRemineralization of white spot lesions has been achieved clinically by applying pastes based on these compounds, and a similar effect to self-applied fluorides has been o …
[Casein Phosphopeptide--Amorphous Calcium Phosphate (CPP-ACP) and Its Effect on Dental Hard Tissues]KW Neuhaus et al. Schweiz Monatsschr Zahnmed 119 (2), 110-6. PMID 19306597. - ReviewDental products with casein phosphopeptide--amorphous calcium phosphate-nanocomplexes (CPP-ACP) are used in several tooth products (toothpastes, chewing gums, mouthrinses …
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The Effect of Antimicrobial Additives on the Properties of Dental Glass-Ionomer Cements: A ReviewT Tüzüner et al. Acta Biomater Odontol Scand 5 (1), 9-21. PMID 30652117. - ReviewAim: The aim of this article is to review the literature on the use of antimicrobial additives in glass-ionomer dental cements. Method: An electronic search …