Recent advancements in matrix-assisted laser desorption/ionization mass spectrometry imaging

Curr Opin Biotechnol. 2017 Feb:43:62-69. doi: 10.1016/j.copbio.2016.09.003. Epub 2016 Sep 28.


Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a robust tool for spatially resolved analysis of biomolecules in situ. Recent advances in high ionization-efficiency MALDI matrices, new matrix deposition procedures, and the development of high spatial-resolution and high sensitivity MS instruments continue to drive new applications of MALDI-MSI, along with other MSI techniques, which allow us to visualize and determine the regio-specific and temporal changes in proteins, peptides, lipids, drug molecules, and metabolites within the tissues, cells and microorganisms. These provide researchers with a new route to the discovery of potential biomarkers of human disease and elucidation of the underlying biology of metabolic regulation, thus bringing our understanding of human health to a new level.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Physiological Phenomena*
  • Humans
  • Imaging, Three-Dimensional / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*