Macrophage effector responses of horses are influenced by expression of CD154

Vet Immunol Immunopathol. 2016 Nov 1;180:40-44. doi: 10.1016/j.vetimm.2016.08.001. Epub 2016 Aug 26.


Reactive intermediates contribute to innate immunity by providing phagocytes with a mechanism of defense against bacteria, viruses and parasites. To better characterize the role of CD154 in the production of reactive intermediates, we cloned and expressed recombinant equine CD154 (reqCD154) in Chinese Hamster Ovary (CHO). In co-culture experiments, CHO cells ectopically expressing reqCD154 elicited superoxide production in monocyte-derived macrophages (MDM). Collectively, our results indicate that regulation of CD154 expression plays a role in innate host defenses.

Keywords: CD154; Equine; Reactive intermediates.

MeSH terms

  • Animals
  • CD40 Antigens / physiology
  • CD40 Ligand / genetics
  • CD40 Ligand / physiology*
  • CHO Cells
  • Coculture Techniques
  • Cricetinae
  • Cricetulus
  • Horses / immunology*
  • Macrophages / immunology*
  • Superoxides / metabolism


  • CD40 Antigens
  • Superoxides
  • CD40 Ligand