Tribulus terrestris Extract Improves Human Sperm Parameters In Vitro

J Evid Based Complementary Altern Med. 2017 Jul;22(3):407-412. doi: 10.1177/2156587216668110. Epub 2016 Sep 30.


Objective: The object of present study was to investigate the effects of direct addition of Tribulus terrestris extract on human sperm parameters.

Design: Semen specimens from 40 healthy men volunteers were divided into 4 groups: one group received no treatment (control group) while the others were incubated with 20, 40, and 50 µg/mL of T terrestris extract (experimental groups). Motility, viability, and DNA fragmentation were assessed in all groups.

Results: The incubation of human semen with 40 and 50 μg/mL of T terrestris extract significantly enhanced total sperm motility, number of progressive motile spermatozoa, and curvilinear velocity over 60 to 120 minutes' holding time ( P < .05 or P < < .01). Furthermore, viability was significantly enhanced by using T terrestris extract ( P < .01).

Conclusions: In vitro addition of the T terrestris extract to human sperm could affect male fertility capacity.

Keywords: DNA fragmentation; Tribulus terrestris; human sperm; motility; viability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Fragmentation / drug effects
  • Humans
  • Male
  • Plant Extracts / pharmacology*
  • Sperm Motility / drug effects
  • Spermatozoa / drug effects*
  • Tribulus*


  • Plant Extracts