Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Jul;22(3):407-412.
doi: 10.1177/2156587216668110. Epub 2016 Sep 30.

Tribulus Terrestris Extract Improves Human Sperm Parameters In Vitro

Affiliations
Free PMC article

Tribulus Terrestris Extract Improves Human Sperm Parameters In Vitro

Sara Khaleghi et al. J Evid Based Complementary Altern Med. .
Free PMC article

Abstract

Objective: The object of present study was to investigate the effects of direct addition of Tribulus terrestris extract on human sperm parameters.

Design: Semen specimens from 40 healthy men volunteers were divided into 4 groups: one group received no treatment (control group) while the others were incubated with 20, 40, and 50 µg/mL of T terrestris extract (experimental groups). Motility, viability, and DNA fragmentation were assessed in all groups.

Results: The incubation of human semen with 40 and 50 μg/mL of T terrestris extract significantly enhanced total sperm motility, number of progressive motile spermatozoa, and curvilinear velocity over 60 to 120 minutes' holding time ( P < .05 or P < < .01). Furthermore, viability was significantly enhanced by using T terrestris extract ( P < .01).

Conclusions: In vitro addition of the T terrestris extract to human sperm could affect male fertility capacity.

Keywords: DNA fragmentation; Tribulus terrestris; human sperm; motility; viability.

Conflict of interest statement

Declaration of Conflicting Interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Figure 1.
Figure 1.
Effects of Tribulus terrestris extract on total motile spermatozoa in human sperm at different concentrations in vitro. Values = mean ± standard error of the mean (SEM), N = 10. *P < .05, **P < .01.
Figure 2.
Figure 2.
Effects of Tribulus terrestris extract on number of progressive motile spermatozoa in human sperm at different concentrations in vitro. Values = mean ± standard error of the mean (SEM), N = 10. *P < .05, **P < .01.
Figure 3.
Figure 3.
Effects of Tribulus terrestris extract on curvilinear velocity (VCL) in human sperm at different concentrations in vitro. Values = mean ± standard error of the mean (SEM), N = 10. *P < .05.
Figure 4.
Figure 4.
Effects of Tribulus terrestris extracts on straight line velocity (VSL) in human sperm at different concentrations in vitro. Values = mean ± standard error of the mean (SEM), N = 10.
Figure 5.
Figure 5.
Effects of Tribulus terrestris extract on viability of human sperm at different concentrations in vitro. Values = mean ± standard error of the mean (SEM), N = 10. *P < .05.

Similar articles

See all similar articles

Cited by 2 articles

References

    1. Kumar N, Singh AK. Trends of male factor infertility, an important cause of infertility: a review of literature. J Hum Reprod Sci. 2015;8:191–196. - PMC - PubMed
    1. Elahi RK, Asl S, Shahian F. Study on the effects of various doses of Tribulus terrestris extract on epididymal sperm morphology and count in rat. Glob Veterin. 2013;10:13–17.
    1. Fekrazad E, Keyhan H, Fekrazad R, Tajik A. Effect of diode lasers on human sperm motility. Acad Res Int. 2014;5:21–25.
    1. Remya M, Sharma RC, Shoaib H, et al. In vitro effect of Aegle marmelos on human sperm motility. J Med Plants Res. 2009;3:1137–1139.
    1. Mukhopadhyay AK, Kumar A. Follicular Growth Ovulation and Fertilization: Molecular and Clinical Basis. Boca Raton, FL: CRC Press; 2002.

Publication types

LinkOut - more resources

Feedback