EIN2-dependent regulation of acetylation of histone H3K14 and non-canonical histone H3K23 in ethylene signalling

Nat Commun. 2016 Oct 3:7:13018. doi: 10.1038/ncomms13018.

Abstract

Ethylene gas is essential for many developmental processes and stress responses in plants. EIN2 plays a key role in ethylene signalling but its function remains enigmatic. Here, we show that ethylene specifically elevates acetylation of histone H3K14 and the non-canonical acetylation of H3K23 in etiolated seedlings. The up-regulation of these two histone marks positively correlates with ethylene-regulated transcription activation, and the elevation requires EIN2. Both EIN2 and EIN3 interact with a SANT domain protein named EIN2 nuclear associated protein 1 (ENAP1), overexpression of which results in elevation of histone acetylation and enhanced ethylene-inducible gene expression in an EIN2-dependent manner. On the basis of these findings we propose a model where, in the presence of ethylene, the EIN2 C terminus contributes to downstream signalling via the elevation of acetylation at H3K14 and H3K23. ENAP1 may potentially mediate ethylene-induced histone acetylation via its interactions with EIN2 C terminus.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / metabolism*
  • Ethylenes / chemistry*
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant*
  • Histones / metabolism*
  • Lysine / chemistry
  • MicroRNAs / metabolism
  • Phenotype
  • Protein Domains
  • Protein Processing, Post-Translational / drug effects
  • Receptors, Cell Surface / metabolism*
  • Seedlings / metabolism
  • Signal Transduction
  • Transcription Factors / metabolism

Substances

  • Arabidopsis Proteins
  • EIN2 protein, Arabidopsis
  • Ethylenes
  • Histones
  • MicroRNAs
  • Receptors, Cell Surface
  • Transcription Factors
  • ethylene
  • Lysine