Transglutaminase-catalyzed amination of pea protein peptides using the biogenic amines histamine and tyramine

J Sci Food Agric. 2017 Jun;97(8):2436-2442. doi: 10.1002/jsfa.8057. Epub 2016 Oct 26.


Background: Biogenic amines (BAs) are produced by the enzymatic decarboxylation of amino acids, and are well-known for their toxicity to humans. This study describes a new method using microbial transglutaminase (MTGase) to covalently link BAs such as histamine (HIS) and tyramine (TYR) to the glutamine residues of alcalase-hydrolyzed pea protein (PPH).

Results: The incubation of PPH and HIS and TYR in the presence of MTGase at 37 °C led to the formation of conjugates, as determined by liquid chromatography, after derivatization with dansyl chloride. Seventy-six % of HIS and 65% of TYR were covalently incorporated to PPH by MTGase. The incubation of PPH and TYR in the presence of MTGase exhibited a 52% DPPH radical scavenging activity at 10 mg mL-1 . Conjugation via MTGase improved the antioxidant status by reducing lipid peroxidation.

Conclusion: This study emphasizes that the application of MTGase can effectively reduce histamine and tyramine content while simultaneously enhancing antioxidative capacity of PPH. © 2016 Society of Chemical Industry.

Keywords: antioxidant capacity; biogenic amines; conjugation; pea protein hydrolysate; transglutaminase.

MeSH terms

  • Amination
  • Aspergillus oryzae / enzymology*
  • Biocatalysis
  • Biogenic Amines / chemistry*
  • Fungal Proteins / chemistry*
  • Histamine / chemistry*
  • Kinetics
  • Pisum sativum / chemistry*
  • Plant Proteins / chemistry*
  • Transglutaminases / chemistry*
  • Tyramine / chemistry*


  • Biogenic Amines
  • Fungal Proteins
  • Plant Proteins
  • Histamine
  • Transglutaminases
  • Tyramine