CD4+ T Cells Coexpressing CD134 (OX40) Harbor Significantly Increased Levels of Human Herpesvirus 6B DNA Following Umbilical Cord Blood Transplantation

J Infect Dis. 2016 Dec 15;214(12):1911-1915. doi: 10.1093/infdis/jiw469. Epub 2016 Oct 4.

Abstract

Human herpesvirus 6B (HHV-6B) commonly reactivates after umbilical cord blood transplantation (UCBT) and is associated with delayed engraftment, fever, rash, and central nervous system dysfunction. Recently, CD134 (OX40) has been implicated as a potential viral entry receptor. We evaluated CD4+CD134+/neg-lo and CD8+CD134+/neg-lo cells at day 28 after UCBT in 20 subjects with previously documented HHV-6 reactivation and persistent viremia. Analysis of CD4+CD134+ cells as compared to CD4+CD134neg-lo cells showed 0.308 versus 0.129 copies of HHV-6B/cell (P = .0002). CD8+CD134+/neg-lo cells contained little to no HHV-6B copies. Following UCBT, CD4+CD134+ cells harbor significantly increased levels of HHV-6B, suggesting that CD134 (OX40) may facilitate viral entry.

Keywords: CD134; HHV-6; OX40; herpesvirus; umbilical cord blood transplantation.

MeSH terms

  • Adult
  • CD4-Positive T-Lymphocytes / chemistry
  • CD4-Positive T-Lymphocytes / virology*
  • CD8-Positive T-Lymphocytes / chemistry
  • CD8-Positive T-Lymphocytes / virology
  • Child
  • Cohort Studies
  • DNA, Viral / analysis*
  • DNA, Viral / genetics
  • DNA, Viral / isolation & purification
  • Female
  • Fetal Blood / virology*
  • Herpesvirus 6, Human / isolation & purification*
  • Humans
  • Infant
  • Male
  • Middle Aged
  • Receptors, OX40 / analysis*
  • Roseolovirus Infections / virology*
  • Transplantation / adverse effects*
  • Young Adult

Substances

  • DNA, Viral
  • Receptors, OX40
  • TNFRSF4 protein, human