Angiotensin-I Converting Enzyme (ACE) Inhibitory and Anti-Hypertensive Effect of Protein Hydrolysate from Actinopyga lecanora (Sea Cucumber) in Rats

Mar Drugs. 2016 Sep 30;14(10):176. doi: 10.3390/md14100176.


Food protein hydrolysates are known to exhibit angiotensin converting enzyme (ACE) inhibitory properties and can be used as a novel functional food for prevention of hypertension. This study evaluated the ACE inhibitory potentials of Actinopyga lecanora proteolysate (ALP) in vivo. The pre-fed rats with ALP at various doses (200, 400, 800 mg/kg body weight) exhibited a significant (p ≤ 0.05) suppression effect after inducing hypertension. To determine the optimum effective dose that will produce maximal reduction in blood pressure, ALP at three doses was fed to the rats after inducing hypertension. The results showed that the 800 mg/kg body weight dose significantly reduced blood pressure without noticeable negative physiological effect. In addition, there were no observable changes in the rats' heart rate after oral administration of the ALP. It was concluded that Actinopyga lecanora proteolysate could potentially be used for the development of functional foods and nutraceuticals for prevention and treatment of hypertension.

Keywords: ACE inhibition; hypertension; normotensive rats; sea cucumber.

MeSH terms

  • Angiotensin-Converting Enzyme Inhibitors / chemistry
  • Angiotensin-Converting Enzyme Inhibitors / pharmacology*
  • Animals
  • Antihypertensive Agents / chemistry
  • Antihypertensive Agents / pharmacology*
  • Blood Pressure / drug effects
  • Dietary Supplements
  • Dose-Response Relationship, Drug
  • Heart Rate / drug effects
  • Male
  • Protein Hydrolysates / chemistry*
  • Protein Hydrolysates / pharmacology*
  • Rats
  • Rats, Inbred SHR
  • Rats, Sprague-Dawley
  • Sea Cucumbers / chemistry*


  • Angiotensin-Converting Enzyme Inhibitors
  • Antihypertensive Agents
  • Protein Hydrolysates