Emerging Field of Cardiomics: High-Throughput Investigations into Transcriptional Regulation of Cardiovascular Development and Disease

Trends Genet. 2016 Nov;32(11):707-716. doi: 10.1016/j.tig.2016.09.002. Epub 2016 Oct 4.

Abstract

Congenital heart defects remain a leading cause of infant mortality in the western world, despite decades of research focusing on cardiovascular development and disease. With the recent emergence of several high-throughput technologies including RNA sequencing, chromatin-immunoprecipitation-coupled sequencing, mass-spectrometry-based proteomics analyses, and the numerous variations of these strategies, investigations into cardiac development have been transformed from candidate-based studies into whole-genome, -transcriptome, and -proteome undertakings. In this review, we discuss several reports that have emerged from our laboratory and others over the past 5 years that emphasize the versatility of large dataset-based investigations of cardiogenic transcription factors, from phenotypic validations and new gene implications to the identification of novel roles of well-studied transcriptional regulators.

Keywords: cardiovascular development; chromatin; congenital heart disease; high-throughput sequencing; proteomics; transcription factor.

Publication types

  • Review

MeSH terms

  • Cardiovascular Diseases / genetics*
  • Cardiovascular System / growth & development*
  • Chromatin / genetics
  • Gene Expression Regulation / genetics*
  • Genome, Human
  • Genomics*
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Proteome / genetics
  • Transcriptome / genetics

Substances

  • Chromatin
  • Proteome