Triacylglycerol compositions of sunflower, corn and soybean oils examined with supercritical CO2 ultra-performance convergence chromatography combined with quadrupole time-of-flight mass spectrometry

Boyan Gao; Yinghua Luo; Weiying Lu; Jie Liu; Yaqiong Zhang; Liangli Lucy Yu

doi:10.1016/j.foodchem.2016.09.099

Triacylglycerol compositions of sunflower, corn and soybean oils examined with supercritical CO₂ ultra-performance convergence chromatography combined with quadrupole time-of-flight mass spectrometry

Food Chem. 2017 Mar 1:218:569-574. doi: 10.1016/j.foodchem.2016.09.099. Epub 2016 Sep 17.

Authors

Boyan Gao¹, Yinghua Luo², Weiying Lu³, Jie Liu⁴, Yaqiong Zhang⁵, Liangli Lucy Yu⁶

Affiliations

¹ Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China; Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA.
² Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA.
³ Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
⁴ Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University, Beijing 100048, China.
⁵ Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China. Electronic address: yqzhang2006@sjtu.edu.cn.
⁶ Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA. Electronic address: lyu5@umd.edu.

PMID: 27719951
DOI: 10.1016/j.foodchem.2016.09.099

Abstract

A supercritical CO₂ ultra-performance convergence chromatography (UPC²) system was utilized with a quadrupole time-of-flight mass spectrometry (Q-TOF MS) to examine the triacylglycerol compositions of sunflower, corn and soybean oils. UPC² provided an excellent resolution and separation for the triacylglycerols, while the high performance Q-TOF MS system was able to provide the molecular weight and fragment ions information for triacylglycerol compound characterization. A total of 33 triacylglycerols were identified based on their elementary compositions and MS² fragment ion profiles, and their levels in the three oils were estimated. The combination of UPC² and Q-TOF MS may determine triacylglycerol compositions for oils and fats, and provide sn-position information for fatty acids, which may be important for food nutritional value and stability.

Keywords: Corn oil; Quadrupole time-of-flight mass spectrometry (Q-TOF MS); Soybean oil; Sunflower oil; Triacylglycerol; Ultra-performance convergence chromatography (UPC(2)).

MeSH terms

Chromatography / methods
Corn Oil / analysis*
Mass Spectrometry / methods
Plant Oils / analysis*
Soybean Oil / analysis*
Sunflower Oil
Triglycerides / analysis*

Substances

Plant Oils
Sunflower Oil
Triglycerides
Soybean Oil
Corn Oil